Free radical scavenging activity and lipoxygenase inhibition. Free radical scavenging activity was determined according to the elimination of dpph radicals and total phenol content. In this study, free radical scavenging activity, total phenolic content, total oxidant status tos, and total antioxidant status tas of methanol ttm and acetone tta extracts of t. Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. These compounds have been described as chainbreaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalizestabilize the resulting phenoxyl radical within their structure. Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants. The % hydroxyl radical scavenging activity of test sample was determined accordingly in comparison with negative control. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents. Free radical inactivator any compound that reacts with free radicals in a biological system, v free radicalinduced damage, and protects against the indirect effects of free radicals produced by ionizing radiation, etc examples ceruloplasmin, cysteine, glutathione, sod, transferrin, vitamins a, c, e, dpenicillamine. The differences between methods conditions and their evaluation are presented.
The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration teac value of 0. Synthesis and dpph radical scavenging activity of 5arylidenen, ndimethylbarbiturates. In order to obtain information about the real antioxidant activity with respect to lipids or food stabilization, it is. The antioxidant activity, reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities were evaluated to determine the.
Antioxidant enzymes and dpphradical scavenging activity. Hydroxyl radical scavenging activity the hydroxyl radical scavenging activity was determined according to the method of beara et al. Invitro antioxidant and free radical scavenging activity. Free radicalscavenging activity of indolic compounds in. Data obtained showed that the extract scavenged superoxide anion radicals ic 501602 gml in a dose dependant manner using. Scavenging definition of scavenging by the free dictionary. Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. Higher % inhibition indicates better scavenging activity. The present study was aimed to investigate the in vitro free radical scavenging activity of various leaf extracts aqueous, methanol, acetone chloroform and petroleum ether of kedrostis foetidissima. Structure radical scavenging activity relationships of flavonoids dragan ami,a, duanka davidoviami,a drago belo,a and nenad trinajstib afaculty of agriculture, the josip juraj strossmayer university, p. The antioxidant activity of the aqueous ethanolic extract of doum leaves, hyphaene thebaica l. The ic 50 values for scavenging of free radicals were 112. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity.
Nitric oxide radical scavenging activity assay figure 3. To measure the antioxidant activity of eps7630 the dpph. Free radical scavenging activities of watersoluble extracts from some natural sources. Hydroxy radical and dpph scavenging activity of crude. Box 180, hr2 zagreb, croatia received march 26, 2002. In this study antioxidant activity was performed by dpph 1, 1diphenyl2picryl hydrazyl radical scavenging method for different extracts of aerial parts like leaves and flowers of ageratum. Dpph free radical scavenging activity of the extracts of the.
Herein, the free radical scavenging ability of puerarin was. All the results were compared with same concentration of ascorbic acid as a standard which showed the highest free radical scavenging activity of 18. Caffeic acid showed significant dpph, hydrogen peroxide and hydroxyl radical scavenging activities of 84. Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was. Comparison of dpph and abts assays for determining. Relationships between free radical scavenging and antioxidant.
The scavenging or inhibiting activity, expressed as mole hydroxyl radical scavenged or reduced per mole of test agent, was calculated as follows. Antioxidant, antimicrobial, and free radical scavenging. Antioxidant and free radical scavenging activity of iron chelators. In addition, aafb melanin exhibited stronger scavenging activities on 2,2 diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. In order to assess the radical scavenging potential of the crude extract, fractions i, ii and the compounds isolated, the reactivity towards the stable free radical dpph was measured at 518 nm by measurement of absorbance decrease of the reaction mixture after 5 hourreaction time. The radicalscavenging activity and total polyphenol content of the samples were highly correlated. Scavenging activity on dpph radical quantitative fig. Polyphenols are widely recognized as potent antioxidants as they can scavenge reactive oxygen species ros.
Indolic compounds are a broad family of substances present in microorganisms, plants and animals. Interaction with dpph showed a similar activity profile. Several methods have been developed to assess the radical scavenging activity. Rphplc method was developed on a phenomenex lunac18. Free radicalscavenging capacity, antioxidant activity and. Pdf in this study antioxidant activity was performed by dpph 1, 1diphenyl2 picryl hydrazyl. In vitro nitric oxide scavenging activity of methanol. Antioxidant and free radical scavenging activity of. The metal chelating activity and reducing power ability of the. The absorbance for reducing power was found to be 0. The antioxidant activity of the memq was evaluated by the. Where the value was less than unity, the result was multiplied by to express the. They are mainly related with tryptophan metabolism, and present particularities that depend on their respective chemical structures. Pdf phenols, a major group of antioxidant phytochemicals, have profound importance due to their biological and free radical scavenging activities.
Hydroxyl radical scavenging activity of flavonoids article pdf available in phytochemistry 269. Quantifying the radical scavenging activity of tea the. The term free radical refers to a molecule that has one or more unpaired electrons. The present study evaluated the in vitro antioxidant and antibacterial activities of various extracts of aerial parts of periploca aphylla and ricinus communis. Pdf antioxidant and free radical scavenging activities. The scavenging coefficient depends on the characteristics of the pollutant e. Pdf synthesis and dpph radical scavenging activity of 5. Radical scavenging capacity of hopderived products dietary antioxidants are believed to be effective in the prevention of oxidative stress related diseases eg. In the search for sources of natural antioxidants and compounds with radical scavenging activity during recent years, some have been found, such as echinacoside in echinaceae root hu and kitts, 2000, anthocyanin espin et al. Mar 29, 2003 indolic compounds are a broad family of substances present in microorganisms, plants and animals. Comparison between the radical scavenging activity and. A mean absorbance with test agent in presence of h 2o2. The free radical scavenger ability of antioxidants can be predicted from standard oneelectron. In vitro antioxidant activities of the plant extract were determined by dpph and no.
A new fenton assay for hydroxyl radical scavengers by. Pdf antioxidant and free radical scavenging activities of some. Antioxidant activity by dpph radical scavenging method of. Dpph radical scavenging activity of various leaf extracts of k. The scavenging ratio is composed of two parameters, precipitation intensity mmhr and a scavenging coefficient smmhr1. Free radical scavenging activity, total phenolic content. Principle of dpph radical scavenging capacity assay. Although puerarin is wellknown for its effective antioxidant activity, there is seldom a systematic theoretical study on its radical scavenging activity. In vitro antioxidant activities of the plant extract were determined by dpph and no scavenging method. Bioactive compounds, radical scavenging, antioxidant. Nitric oxide radical scavenging activity sodium nitroprusside 5mm, 1ml in phosphate buffer saline pbs 0. Pdf antioxidant activity by dpph radical scavenging method of. Invitro antioxidant and free radical scavenging activity of. Calculations the data were subjected to routine statistical analysis.
Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh. Evaluation of free radical scavenging activity of various. Selective abts and dpph radical scavenging activity of. The scavenging activity was calculated as a percentage of the radical reduction. The solution is diluted with water to achieve an absorbance of 0. Antioxidant and free radical scavenging activities of some fruits article pdf available in journal of complementary and integrative medicine 81 january 2011 with 430 reads how we measure. Antioxidant and free radical scavenging activity of spondias.
Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186. In vitro antioxidant and free radical scavenging activity of. The study indicates that the phenolic group is essential for the free radical scavenging activity and presence of methoxy group further increases the activity. Superoxide anion radical scavenging activity biology essay. Pdf antioxidant activity by dpph radical scavenging. Box 719, hr31107 osijek, croatia bthe rugjer bokovi institute, p. Scavenging activity % 1absorbance sample absorbance control. Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable 1, 1 diphenyl 2picrylhyorazyl dpph free radical according to the method described by brandwilliams et al22 with slight modifications. Free radical scavenging activity of crude extracts and 4.
Measurement of the dpphradical scavenging activity. Antioxidant and free radical scavenging activities of. The percentages of radical scavenging property of the ethanolic, aqueous extract and parthenolide in this assay were 63. Free radical scavengers definition of free radical. The antioxidant activity of the aerial part extract of m. To compare the antioxdant activity we used abts 2,2azinobis 3ethylbenzothiazoline6sulfonic acid diammonium radical scavenging assay which is applicable for both lipophilic and hydrophilic antioxidants. Structureradical scavenging activity relationships of. Thirteen hypericum species growing in bulgaria were investigated for free radicalscavenging activity, antioxidant activity, total tannins and total flavonoids contents. Three natural curcuminoids curcumin cas 458377, demethoxycurcumin, bisdemethoxycurcumin and acetylcurcumin.
All the extracts showed different levels of dpph radical scavenging activity over the range of 210 mgml concentration and the ec50 value of aqkf, mkf, ackf, ckf and pekf was found to be 4. International journal of applied science and technology vol. Free radical scavenging activity of crude extracts and 4 bioline. Invitro antioxidant and free radical scavenging activity of aqueous. Determination of free radical scavenging activity of plant. Chrysin, rutin and quercetin were run to explore the effect of. Free radical scavenging activity and cytotoxic effect of. Free radical scavenging activity screening of medicinal. They are very unstable and react quickly with other compounds. These results suggest that tpl had the highest radical. Methanolic extracts from the hypericum species were analyzed for radical scavenging and antioxidant activities using dpph, abts free radicals, total antioxidant activity and inhibition of lipid.
This makes them very unstable, and they move through the bloodstream, taking electrons from other cells or giving away unpaired ones. The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11. In vitro antioxidant and free radical scavenging activity. Pdf radical scavenging activity and antioxidant capacity of. The total phenolic content of the extracts was found to be higher in a.
Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. Comparation of free radical scavenging activity between. The results of the assay are given in the figure 1. Phytochemical screening and free radical scavenging. The dpph radicalscavenging activity, peroxy radicalscavenging activity and total polyphenol content of the dairy products containing coffee, cocoa, tea, vegetables or fruits were higher than those of cows milk. Based on dpph and hydroxyl radical scavenging activity, tpl showed strong scavenging activity 91. Many diseases are associated with oxidative stress caused by free radicals. A free radical scavenger is a vitamin, mineral, or enzyme that is able to destroy free radicals. The free radical scavenger ability of antioxidants can be predicted from standard oneelectron potentials. Phytochemical screening and free radical scavenging activity. It was determined the effect of methods conditions by ruggedness testing of methods. Puerarin is a cglycoside of daidzein, one of the major bioactive ingredients isolated from the root of pueraria lobata, which has a wide spectrum of pharmacological effects. Chemical composition and radical scavenging activity of. Oxygen radical, nitric oxide and human inflammatory joints disease.
Determination of dpph free radical scavenging activity by rp. Onehalf milliliter of the solution was mixed with 0. Hydroxyl radical scavenging activity assay figure 4. Superoxide anions were generated in a nonenzymatic phenazinemetho. Dpph free radical scavenging activity of the extracts of. Free radical scavenger definition of free radical scavenger. Au515 a0a1 a0ka1k where au515 is the antiradical activity of the extract, a0 the absorbance of the sample at the beginning of the reaction 0 min, a1 the absorbance of the sample after incubation times 20120 sec of the reaction.
Comparative study of abts radical scavenging activity and. Pdf radical scavenging activity and antioxidant capacity. Reducing power assay 0 20 40 60 80 100 0 100 200 300 400 500 % concentration gml dpph radical scavanging activity ascorbic acid sida acuta 0 10 20 30 40 50 60 70 90 0 100 200. Free radicals, formed in the course of normal cellular respiration and metabolism, and more abundantly under the influence of certain environmental chemicals and sunlight, have been inculpated in various types of tissue damage, particularly those involved in atherosclerosis, the aging process, and the development of cancers. The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Pdf hydroxyl radical scavenging activity of flavonoids. In vitro free radical scavenging activities of the extracts were assessed against dpph and hydroxyl radicals. The antioxidant activity of the catechins found in white tea is much greater than that of ferulic acid, as determined using the spin trap method and electron paramagnetic resonance spectroscopy free radicals are formed when weak bonds within a molecule break, leaving an unpaired electron. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. The most important members of the family are the plant hormone, indole3acetic acid, and the animal hormone, melatonin. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern.
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